Histone H2A type 3 Polyklonaler Antikörper

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• KD/KO Validated

Histone H2A type 3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10445-1-AP

Synonyme

HIST3H2A, histone, histone cluster 3, H2a, Histone H2A, Histone H2A type 3

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Galerie der Validierungsdaten

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  WB analysis of human brain using 10445-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10445-1-AP (Histone H2A type 3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 10445-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10445-1-AP (Histone H2A type 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10445-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10445-1-AP (HIST3H2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, fetales humanes Hirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

10445-1-AP bindet in WB, IP, IF, IHC, ELISA Histone H2A type 3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H2A type 3 fusion protein Ag0374
• Vollständiger Name: histone cluster 3, H2a
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14-18 kDa
• GenBank-Zugangsnummer: BC001193
• Gene symbol: Histone H2A type 3
• Gene ID (NCBI): 92815
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone H2A type 3, it is expected to be located in the nucleus, and the protein is enriched in the brain tissue. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. The molecular weight of Histone H2A type 3 is 14 kDa.

Publikationen

Species	Application	Title
human,mouse	WB,IP,IF	Nucleic Acids Res; H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor.; Authors - Libo Su ; KO Validated
mouse	WB	Nucleic Acids Res; Brain-specific deletion of histone variant H2A.z results in cortical neurogenesis defects and neurodevelopmental disorder.; Authors - Tianjin Shen
mouse	IHC	EMBO Rep; MacroH2A1.2 deficiency leads to neural stem cell differentiation defects and autism-like behaviors.; Authors - Hongyan Ma
human	WB	BMC Cancer; HIST3H2A promotes the progression of prostate cancer through inhibiting cell necroptosis; Authors - Lihong Yang