Di-Methyl-Histone H3 (Lys36) Polyklonaler Antikörper

Di-Methyl-Histone H3 (Lys36) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29202-1-AP

Synonyme

H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

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Galerie der Validierungsdaten

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  WB analysis using 29202-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29202-1-AP (HIST1H3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29202-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29202-1-AP (Di-Methyl-Histone H3 (Lys36) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  ELISA experiment of peptide using 29202-1-AP

  Di-Methyl-Histone H3(Lys36) antibody (29202-1-AP) tested by ELISA. HIST1H3A(31-43aa), HIST1H3AMe1(31-43aa), HIST1H3AMe2(31-43aa), HIST1H3AMe3(31-43aa) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of HIST1H3AMe2 antibody (29202-1-AP). Bound antibodies were detected with HRP conjugated anti-rabbit IgG followed by incubation with HRP substrate and then measuring the resulting absorbance at 450 nm.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12-Zellen, HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in ELISA: peptide

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:32000-1:128000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29202-1-AP bindet in WB, ELISA Di-Methyl-Histone H3 (Lys36) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Beobachtetes Molekulargewicht: 15-18 kDa
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).NSD enzymes exhibit an auto-inhibitory state that is relieved by nucleosome engagement, allowing for H3K36 di-methylation catalysis (PMID:22099308).