HIST1H1B Polyklonaler Antikörper

HIST1H1B Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18093-1-AP

Synonyme

H1, H1.5, H1F5, HIST1H1B, histone cluster 1, H1b, Histone H1.5, Histone H1a

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Galerie der Validierungsdaten

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  WB analysis using 18093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18093-1-AP (HIST1H1B antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 18093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18093-1-AP (HIST1H1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (HIST1H1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (HIST1H1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 18093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18093-1-AP (HIST1H1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 18093-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using HIST1H1B antibody (18093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:30000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18093-1-AP bindet in WB, IHC, IF, ELISA HIST1H1B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIST1H1B fusion protein Ag12818
• Vollständiger Name: histone cluster 1, H1b
• Berechnetes Molekulargewicht: 226 aa, 23 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC101581
• Gene symbol: HIST1H1B
• Gene ID (NCBI): 3009
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Histone Cluster 1 H1 Family Member B (HIST1H1B) is a member of the Histone H1/H5 protein family. HIST1H1B gene has been found to be frequently mutated in colorectal cancer and follicular lymphomas. HIST1H1B overexpression contributes to tumor aggressiveness. HIST1H1B expression is upregulated in breast cancer and predicts poor prognosis(PMID: 34746019).