HIRA Rekombinanter Antikörper

HIRA Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240006F8

Kat-Nr. : 83194-4-RR

Synonyme

240006F8, HIR, HIRA/HIR, Protein HIRA

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  WB analysis using 83194-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83194-4-RR (HIRA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 83194-4-RR

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug HIRA Recombinant antibody (83194-4-RR, Clone:240006F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83194-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83194-4-RR against Human HIRA were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche Detektion in FC (Intra): K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83194-4-RR bindet in WB, ELISA HIRA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HIRA fusion protein Ag34439
• Vollständiger Name: HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 112 kDa
• Beobachtetes Molekulargewicht: 112 kDa
• GenBank-Zugangsnummer: NM_003325
• Gene symbol: HIRA
• Gene ID (NCBI): 7290
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIRA protein is encoded by a gene within a region of human chromosome 22q11.2 deleted in most patients with the DiGeorge syndrome, a developmental disorder, and was named for its amino acid sequence homology to two S. cerevisiae proteins, Hir1p and Hir2p. HIRA is critical in a specific chromatin assembly pathway that takes place independently of DNA synthesis (PMID: 12049744). HIRA complex associates with various proteins, such as transcription factors, RNA pol II, Prohibitin (PHB), and replication protein A (RPA) suggests that the targeting of the complex at specific locations may exploit additional structural properties and mechanisms (PMID: 30082790).