HHIP Polyklonaler Antikörper

HHIP Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 11654-1-AP

Synonyme

hedgehog interacting protein, HHIP, HIP

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11654-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11654-1-AP (HHIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11654-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11654-1-AP (HHIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

11654-1-AP bindet in WB, ELISA HHIP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HHIP fusion protein Ag2241
• Vollständiger Name: hedgehog interacting protein
• Berechnetes Molekulargewicht: 700 aa, 79 kDa
• GenBank-Zugangsnummer: BC025311
• Gene symbol: HHIP
• Gene ID (NCBI): 64399
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HHIP is a member of the hedgehog-interacting protein (HHIP) family. The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. The HHIP encoded by this gene is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH. Two single nucleotide polymorphisms (SNPs) near this gene are significantly associated with risk of chronic obstructive pulmonary disease (COPD). A single nucleotide polymorphism in this gene is also strongly associated with human height.

Publikationen

Species	Application	Title
mouse	WB	J Mol Cell Biol; Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.; Authors - Yuwei Li
human	WB	Int Immunopharmacol; Wogonin inhibits the growth of HT144 melanoma via regulating hedgehog signaling-mediated inflammation and glycolysis.; Authors - Ling Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chaoqun (Verified Customer) (07-30-2019)

For IF analysis of paraffin embedded mouse lung section, it did not work well.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Paraffin embedded mouse lung section