HEXA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HEXA Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (2)

Kat-Nr. : 11317-1-AP

Synonyme

TSD, N-acetyl-beta-glucosaminidase subunit alpha, Hexosaminidase subunit A, EC:3.2.1.52, Beta-N-acetylhexosaminidase subunit alpha

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Galerie der Validierungsdaten

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  WB analysis using 11317-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11317-1-AP (HEXA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11317-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11317-1-AP (HEXA antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of PC-3 using 11317-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 11317-1-AP (HEXA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

11317-1-AP bindet in WB, IHC, IF/ICC, ELISA HEXA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HEXA fusion protein Ag1854
• Vollständiger Name: hexosaminidase A (alpha polypeptide)
• Berechnetes Molekulargewicht: 529 aa, 60 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC018927
• Gene symbol: HEXA
• Gene ID (NCBI): 3073
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta-hexosaminidase subunit alpha (HEXA) is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).

Publikationen

Species	Application	Title
mouse	WB	Immunity; The phagosomal proteome in interferon-gamma-activated macrophages.; Authors - Matthias Trost
mouse	IF	J Immunol; VAMP8 is essential in anaphylatoxin-induced degranulation, TNF-alpha secretion, peritonitis, and systemic inflammation.; Authors - Pushparaj Peter N PN
human	IHC,IF,WB	Front Oncol; Microglia-Specific Expression of HEXA and HEXB Leads to Poor Prognosis in Glioblastoma Patients.; Authors - Mengxian Jia ; KD Validated
human	WB	Nat Commun; Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis; Authors - Sebastian Boland
human	IHC	Proc Natl Acad Sci U S A; A small-molecule drug inhibits autophagy gene expression through the central regulator TFEB; Authors - Yuqi Lin
mouse,rat	IF	Nat Commun; Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents; Authors - Yachao He

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joshua (Verified Customer) (01-26-2023)

Did not detect expected mature HEXA ~54kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:20,000
• Cell Tissue Type: HEK293T

HEXA Antibody Western Blot validation (1:20,000 dilution) in HEK293T (Cat no:11317-1-AP)

FH

Georgina (Verified Customer) (09-15-2022)

Good to have the chance to buy a sample to try out an experiment. The antibody worked.

• Applications: Western Blot