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  • KD/KO Validated

HECTD3 Polyklonaler Antikörper

HECTD3 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11487-1-AP

Synonyme

HECT domain containing 3, HECTD3, RP11 69J16.1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMCF-7-Zellen, MDA-MB-453s-Zellen
Erfolgreiche IPMDA-MB-453s-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:3000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11487-1-AP bindet in WB, IP, IF, CoIP, ELISA HECTD3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HECTD3 fusion protein Ag1998
Vollständiger Name HECT domain containing 3
Berechnetes Molekulargewicht 861 aa, 97 kDa
Beobachtetes Molekulargewicht 97 kDa
GenBank-ZugangsnummerBC019105
Gene symbol HECTD3
Gene ID (NCBI) 79654
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HECTD3(HECT domain-containing protein 3) may be involved in the regulation of mitotic metaphase/anaphase transition. It can specifically ubiquitinate TRIOBP and target it for proteasomal degradation and it can also mediates the ubiquitination of STX8. HECTD3 is a new oncogenic E3 ligase in human prostate cancer.( Li Y, etc. 2008) HECTD3 may facilitate cell cycle progression via regulating ubiquitination and degradation of Tara(PMID:18194665). It has 2 isoforms produced by alternative splicing.

Protokolle

Produktspezifische Protokolle
WB protocol for HECTD3 antibody 11487-1-APProtokoll herunterladen
IP protocol for HECTD3 antibody 11487-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouse,humanWB

Nat Commun

E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3.

Authors - Peng Gao
humanWB

Front Oncol

uc.77- Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation.

Authors - Zhijian Zheng
humanWB

Cell Death Discov

HECTD3 promotes gastric cancer progression by mediating the polyubiquitination of c-MYC.

Authors - Guanghui Zhang
  • KD Validated
human,mouseWB,IP

Br J Cancer

HECTD3 regulates the tumourigenesis of glioblastoma by polyubiquitinating PARP1 and activating EGFR signalling pathway

Authors - Guanghui Zhang
  • KD Validated
humanCoIP

Cell Death Dis

Radiation induces IRAK1 expression to promote radioresistance by suppressing autophagic cell death via decreasing the ubiquitination of PRDX1 in glioma cells

Authors - Jing Li
mouseIF

Cell Death Dis

HECTD3 inhibits NLRP3 inflammasome assembly and activation by blocking NLRP3-NEK7 interaction

Authors - Zhuo Cheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Wei (Verified Customer) (01-23-2020)

Good, single band

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C2C12