HDAC2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HDAC2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

74

Rezensionen (3)

Kat-Nr. : 12922-3-AP

Synonyme

YAF1, RPD3, histone deacetylase 2, HD2, EC:3.5.1.98

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Galerie der Validierungsdaten

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  WB analysis using 12922-3-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12922-3-AP (HDAC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12922-3-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12922-3-AP (HDAC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 12922-3-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12922-3-AP (HDAC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12922-3-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12922-3-AP (HDAC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12922-3-AP

  IP result of anti-HDAC2 (IP:12922-3-AP, 4ug; Detection:12922-3-AP 1:20000) with HEK-293 cells lysate 960 ug.

• 

  IHC staining of mouse brain using 12922-3-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12922-3-AP (HDAC2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12922-3-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12922-3-AP (HDAC2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12922-3-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC2 antibody (12922-3-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 12922-3-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC2 antibody (12922-3-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C6-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Nierengewebe, L02-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Rattenlebergewebe, Rattennierengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 14 publications below
• WB: See 63 publications below
• IHC: See 13 publications below
• IF: See 6 publications below
• IP: See 8 publications below
• CoIP: See 3 publications below
• ChIP: See 3 publications below

Produktinformation

12922-3-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA HDAC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HDAC2 fusion protein Ag3607
• Vollständiger Name: histone deacetylase 2
• Berechnetes Molekulargewicht: 458 aa, 52 kDa; 488 aa,55 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC031055
• Gene symbol: HDAC2
• Gene ID (NCBI): 3066
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.

Publikationen

Species	Application	Title
rat	IP	Cell Metab; Sphingolipid metabolism controls mammalian heart regeneration; Authors - Xiaoqian Ji
human	CoIP	J Clin Invest; SAP30 promotes breast tumor progression by bridging the transcriptional corepressor SIN3 complex and MLL1; Authors - Lei Bao
human	WB	Acta Pharm Sin B; Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.; Authors - Xin Sun
mouse	WB	J Clin Invest; Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.; Authors - Shanshan Zhong
mouse	IP	Nat Commun; The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.; Authors - Xinzhi Li
mouse	WB,IP	J Exp Med; ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.; Authors - Longfei Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhongwen (Verified Customer) (09-25-2023)

There are two bands close to each other. Maybe they are different isoforms. But the quality is quite good.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

HDAC2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:12922-3-AP)

FH

Iram (Verified Customer) (09-17-2020)

HDAC2 antibody is very good. Giving very clear bands and very good staining in tissue sections

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
• Cell Tissue Type: Human colon cancer

FH

Dipen (Verified Customer) (09-04-2020)

Excellent antibody. One single clean band at the correct molecular weight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neuron

HDAC2 Antibody Western Blot validation (1:1000 dilution) in Neuron (Cat no:12922-3-AP)