HDAC1-specific Polyklonaler Antikörper

HDAC1-specific Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16160-1-AP

Synonyme

GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 16160-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16160-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 16160-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16160-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 16160-1-AP

  IP Result of anti-HDAC1-specific (IP:16160-1-AP, 4ug; Detection:16160-1-AP 1:500) with mouse testis tissue lysate 4000ug.

• 

  IF Staining of HepG2 using 16160-1-AP

  Immunofluorescent analysis of HepG2 cells using 16160-1-AP (HDAC1-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Maushirngewebe, Maus-Thymusgewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16160-1-AP bindet in WB, IP, IF,ELISA HDAC1-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone deacetylase 1
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_004964
• Gene symbol: HDAC1
• Gene ID (NCBI): 3065
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is a rabbit polyclonal antibody raised against a peptide mapping near the N-terminus of human HDAC1 and is specific to HDAC1. It does not react with other HDACs.