HBS1L Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HBS1L Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10359-1-AP

Synonyme

HSPC276, HBS1, HBS 1, ERFS, EF 1a

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 10359-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10359-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10359-1-AP

  WB result of HBS1L antibody (10359-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells.

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  WB analysis of Jurkat using 10359-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10359-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 10359-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10359-1-AP

  IP result of anti-HBS1L (IP:10359-1-AP, 4ug; Detection:10359-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 10359-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10359-1-AP (HBS1L antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of HepG2 using 10359-1-AP

  Immunofluorescent analysis of HepG2 cells, using HBS1L antibody 10359-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A2780-Zellen, HEK-293-Zellen, Maushirngewebe, SKOV-3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below

Produktinformation

10359-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA HBS1L und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HBS1L fusion protein Ag0388
• Vollständiger Name: HBS1-like (S. cerevisiae)
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC001465
• Gene symbol: HBS1L
• Gene ID (NCBI): 10767
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.This antibody specifically recognizes the 75kd human HBS1L protein.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Deep sequencing shows multiple oligouridylations are required for 3' to 5' degradation of histone mRNAs on polyribosomes.; Authors - Michael K Slevin
mouse	WB	Elife; Defects in translation-dependent quality control pathways lead to convergent molecular and neurodevelopmental pathology.; Authors - Markus Terrey
human	WB	Biochem J; Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation.; Authors - John Rp Knight
human	WB	J Biol Chem; RNA exosome complex regulates stability of Hepatitis B Virus's X-mRNA transcript in a Non-Stop mediated (NSD) RNA quality control mechanism.; Authors - Hussein H Aly ; KD Validated
mouse	WB	PLoS Genet; Mammalian Hbs1L deficiency causes congenital anomalies and developmental delay associated with Pelota depletion and 80S monosome accumulation.; Authors - Amy E O'Connell ; KO Validated
mouse	WB	Immunity; Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity; Authors - Xiurong Wu ; KO Validated