HAT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HAT1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (2)

Kat-Nr. : 11432-1-AP

Synonyme

KAT1, EC:2.3.1.48

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11432-1-AP

  WB result of HAT1 antibody (11432-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAT1 transfected HEK-293 cells.

• 

  WB analysis using 11432-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11432-1-AP (HAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11432-1-AP

  IP result of anti-HAT1 (IP:11432-1-AP, 4ug; Detection:11432-1-AP 1:500) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human lymphoma using 11432-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11432-1-AP (HAT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse ovary using 11432-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using HAT1 antibody (11432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11432-1-AP

  Immunofluorescent analysis of HepG2 cells, using HAT1 antibody 11432-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, HEK-293-Zellen, MDA-MB-231-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Eierstockgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

11432-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA HAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HAT1 fusion protein Ag1952
• Vollständiger Name: histone acetyltransferase 1
• Berechnetes Molekulargewicht: 419 aa, 50 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC018682
• Gene symbol: HAT1
• Gene ID (NCBI): 8520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
human	WB	EMBO Rep; Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.; Authors - Guang Yang ; KD Validated
human	WB	Mol Cell Proteomics; Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).; Authors - Heguo Yu
rat	IHC	Cereb Cortex; New Cortical Neurodegenerative Pathways in the Hypertensive Rat Brain.; Authors - Ben Shabat Moti
mouse	WB	Alcohol; Hepatic COX-2 expression protects mice from an alcohol-high fat diet-induced metabolic disorder by involving protein acetylation related energy metabolism.; Authors - Minjie Chen
human	IF	Biochem Biophys Res Commun; Identification and characterization of an endoplasmic reticulum localization motif.; Authors - Yong-Wei Nie

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kathryn (Verified Customer) (04-22-2022)

This antibody works very well to stain nuclei of developed oocytes in the mouse ovary.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse ovary sections

HAT1 Antibody Immunofluorescence validation (1:100 dilution) in Mouse ovary sections (Cat no:11432-1-AP)

FH

Leonardo (Verified Customer) (05-29-2019)

The antibody works well in a good range of sample (5-40 ug) in both cell lines I tested (MDA-MB-231 and HCC1806). I definitely recommend.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: breast cancer cell lines

HAT1 Antibody Western Blot, validation (1:1000 dilution) in breast cancer cell lines (Cat no:11432-1-AP)