H3F3B Polyklonaler Antikörper

H3F3B Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10265-1-AP

Synonyme

H3 histone, family 3B (H3.3B), H3.3A, H3.3B, H3F3, H3F3A, H3F3B, histone, Histone H3.3



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

10265-1-AP bindet in WB, ELISA H3F3B und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen H3F3B fusion protein Ag0326
Vollständiger Name H3 histone, family 3B (H3.3B)
Berechnetes Molekulargewicht 15 kDa
Beobachtetes Molekulargewicht 17 kDa
GenBank-ZugangsnummerBC001124
Gene symbol H3F3B
Gene ID (NCBI) 3021
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. H3F3B is one replacement histone [PMID: 8586426]. It constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Thereby it has a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability [PMID: 14718166, 15776021].

Protokolle

Produktspezifische Protokolle
WB protocol for H3F3B antibody 10265-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Commun Signal

Reciprocal regulation between RACGAP1 and AR contributes to endocrine therapy resistance in prostate cancer

Authors - Jiajia Wang