Glucagon Monoklonaler Antikörper

Glucagon Monoklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

2A8G1

Kat-Nr. : 67286-1-Ig

Synonyme

GCG, GLP 2, GLP1, GLP2, glucagon, GRPP, GRPP Oxyntomodulin, OXM

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Galerie der Validierungsdaten

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  IHC staining of human pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000(under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 67286-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human pancreas using 67286-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 67286-1-Ig (Glucagon antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The B cells of the islets of Langerhans was labelled in red with 15848-1-AP (Insulin antibody).

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  IF Staining of mouse pancreas using 67286-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Glucagon antibody (67286-1-Ig, Clone: 2A8G1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), INS antibody (15848-1-AP, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Pankreasgewebe, Maus-Pankreasgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:2000-1:15000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 3 publications below

Produktinformation

67286-1-Ig bindet in IHC, IF, ELISA Glucagon und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Glucagon fusion protein Ag10629
• Vollständiger Name: glucagon
• Berechnetes Molekulargewicht: 180 aa, 21 kDa
• GenBank-Zugangsnummer: BC005278
• Gene symbol: GCG
• Gene ID (NCBI): 2641
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucagon is a 29-amino acid peptide hormone secreted from the pancreatic alpha cells with a powerful stimulatory effect on hepatic glucose production acting to increase plasma glucose levels. Glucagon is best known as the counter-regulatory hormone to INS, and normal glucose homeostasis depends largely on the balanced secretion of INS and glucagon from the pancreatic beta and alpha cells, respectively.The regulation of glucose metabolism by glucagon is mediated by its direct action on the peripheral tissues such as the liver and also by the brain. Glucagon is also released postprandially in a transient manner, which was shown to be involved in inhibition of food intake via reduction of meal size.

Publikationen

Species	Application	Title
mouse	IF	Food Chem X; Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice.; Authors - Jingyu Fang
mouse	IF	Mol Cell Endocrinol; Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.; Authors - Xinghong Guo
mouse	IF	NPJ Biofilms Microbiomes; Bacteroides ovatus accelerates metformin-induced vitamin B12 deficiency in type 2 diabetes patients by accumulating cobalamin; Authors - Manyun Chen
human		Mol Metab; Long non-coding RNA LncCplx2 regulates glucose homeostasis and pancreatic β cell function; Authors - Linlin Wang