Gamma Catenin Monoklonaler Antikörper

Gamma Catenin Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Hund, Ratte

Anwendung

WB

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A5D10

Kat-Nr. : CL488-66445

Synonyme

ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin

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Galerie der Validierungsdaten

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  WB analysis using CL488-66445

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66445 (Gamma Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using CL488-66445

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66445 (Gamma Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, T-47D-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66445 bindet in WB Gamma Catenin und zeigt Reaktivität mit Hausschwein, human, Hund, Ratten

• Getestete Reaktivität: Hausschwein, human, Hund, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gamma Catenin fusion protein Ag1627
• Vollständiger Name: junction plakoglobin
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC011865
• Gene symbol: JUP
• Gene ID (NCBI): 3728
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.