GTPBP4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GTPBP4 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 13897-1-AP

Synonyme

NGB, GTP-binding protein NGB, GTP-binding protein 4, GTP binding protein NGB, GTP binding protein 4

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Galerie der Validierungsdaten

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  WB analysis using 13897-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13897-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 13897-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13897-1-AP

  IP result of anti-GTPBP4 (IP:13897-1-AP, 4ug; Detection:13897-1-AP 1:800) with mouse testis tissue lysate 4000ug.

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  IF Staining of HeLa using 13897-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13897-1-AP (GTPBP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Nierengewebe, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13897-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GTPBP4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, drosophila, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GTPBP4 fusion protein Ag4859
• Vollständiger Name: GTP binding protein 4
• Berechnetes Molekulargewicht: 634 aa, 74 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC038975
• Gene symbol: GTPBP4
• Gene ID (NCBI): 23560
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target.

Publikationen

Species	Application	Title
mouse	WB	Genes Dev; Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome.; Authors - Andrew J Finch
Drosophila	WB,IF	Proc Natl Acad Sci U S A; A genome-scale protein interaction profile of Drosophila p53 uncovers additional nodes of the human p53 network.; Authors - Lunardi Andrea A ; KD Validated
human	WB,IHC	Biochem Biophys Res Commun; Up-regulation of GTPBP4 in colorectal carcinoma is responsible for tumor metastasis.; Authors - Haitao Yu ; KD Validated
human	WB	Biomed Res Int; Determining the Clinical Value and Critical Pathway of GTPBP4 in Lung Adenocarcinoma Using a Bioinformatics Strategy: A Study Based on Datasets from The Cancer Genome Atlas.; Authors - Zhiqian Zhang ; KD Validated
human	WB	Cell Rep; UTP18-mediated p21 mRNA instability drives adenoma-carcinoma progression in colorectal cancer; Authors - Meng Pan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

kaosheng (Verified Customer) (11-11-2019)

This antibody detects a very clear and specific band in polysome profiling fractions of human TF1 cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: TF1