TFIIE Beta Polyklonaler Antikörper

TFIIE Beta Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11596-1-AP

Synonyme

GTF2E2, TFIIE-beta, TFIIE B, TF2E2

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 11596-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 11596-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11596-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A375 using 11596-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using TFIIE Beta antibody (11596-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, A375-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

11596-1-AP bindet in WB, IF/ICC, ELISA TFIIE Beta und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFIIE Beta fusion protein Ag2136
• Vollständiger Name: general transcription factor IIE, polypeptide 2, beta 34kDa
• Berechnetes Molekulargewicht: 291 aa, 33 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC030572
• Gene symbol: GTF2E2
• Gene ID (NCBI): 2961
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The general transcription factor IIE (TFIIE) is an essential component of the eukaryotic RNA polymerase II initiation complex, which plays pivotal roles in initiation of protein-coding gene transcription (PMID: 7829083). Transcription initiation factor IIE comprises two subunits, TFIIE-alpha and TFIIE-beta, two chains of which together form a tetramer. TFIIE-beta is encoded by GTF2E2 gene and contains TFIIE beta-DNA binding domain. It mainly functions to recruit TFIIH to the initiation complex and stimulate RNA polymerase II kinase activity (PMID: 10792464).

Publikationen

Species	Application	Title
mouse	WB	Nucleic Acids Res; Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation.; Authors - De Cegli Rossella R
human	WB	Cancer Gene Ther; GTF2E2 downregulated by miR-340-5p inhibits the malignant progression of glioblastoma; Authors - Xiaolong Qiao