GSTP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GSTP1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

31

Rezensionen (1)

Kat-Nr. : 15902-1-AP

Synonyme

DFN7, FAEES3, Glutathione S transferase P, glutathione S transferase pi 1, GST class pi, GST3, GSTP1, GSTP1 1, PI

---

Galerie der Validierungsdaten

• 

  WB analysis using 15902-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15902-1-AP (GSTP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15902-1-AP

  WB result of GSTP1 antibody (15902-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTP1 transfected HeLa cells.

• 

  WB analysis of human brain using 15902-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15902-1-AP (GSTP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15902-1-AP

  IP Result of anti-GSTP1 (IP:15902-1-AP, 3ug; Detection:15902-1-AP 1:800) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human skin using 15902-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15902-1-AP (GSTP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 15902-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15902-1-AP (GSTP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 15902-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15902-1-AP (GSTP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15902-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15902-1-AP (GSTP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 15902-1-AP

  Immunofluorescent analysis of HepG2 cells using 15902-1-AP (GSTP1 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 15902-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug GSTP1 antibody (15902-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Hirngewebe, Jurkat-Zellen, K-562-Zellen, Mausherzgewebe, Maushirngewebe, PC-3-Zellen, Rattenherzgewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

15902-1-AP bindet in WB, IP, IHC, IF, FC, ELISA GSTP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTP1 fusion protein Ag8731
• Vollständiger Name: glutathione S-transferase pi 1
• Berechnetes Molekulargewicht: 210 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23-28 kDa
• GenBank-Zugangsnummer: BC010915
• Gene symbol: GSTP1
• Gene ID (NCBI): 2950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glutathione S-transferase P (GSTP1) conjugates reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. It is involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276).

Publikationen

Species	Application	Title
human	WB,IP	Mol Cell; SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation; Authors - Wenxin Zhang ; KD Validated
mouse	WB	Redox Biol; GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux; Authors - Xiaoxiao Ji ; KD Validated
mouse	IF	J Cell Biol; TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.; Authors - Wan Yun Ho
mouse	WB	Part Fibre Toxicol; Protein target identification and toxicological mechanism investigation of silver nanoparticles-induced hepatotoxicity by integrating proteomic and metallomic strategies.; Authors - Ming Xu
mouse	WB	Cell Death Dis; Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.; Authors - Cheng Wang
human	IHC	Oncotarget; Prognostic significance of GSTP1 in patients with triple negative breast cancer.; Authors - Guanglei Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

James (Verified Customer) (11-30-2022)

Works pretty well.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

GSTP1 Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:15902-1-AP)

Verwandte Produkte

GSTM1 Polyclonal antibody

12412-1-AP

GSTT1 Polyclonal antibody

15838-1-AP

GSTM3 Polyclonal antibody

15214-1-AP