GSTA2 Polyklonaler Antikörper

GSTA2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 10505-1-AP

Synonyme

Glutathione S transferase A2, GST class alpha member 2, GST gamma, GST HA subunit 2, GST2, GSTA2, GSTA2 2, GTA2, GTH2

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Galerie der Validierungsdaten

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  WB analysis of human testis using 10505-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10505-1-AP (GSTA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10505-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10505-1-AP (GSTA2 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 10505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10505-1-AP (GSTA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10505-1-AP (GSTA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10505-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10505-1-AP (GSTA2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse brain using 10505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10505-1-AP (GSTA2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10505-1-AP (GSTA2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

10505-1-AP bindet in WB, IHC, ELISA GSTA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTA2 fusion protein Ag0781
• Vollständiger Name: glutathione S-transferase alpha 2
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC002895
• Gene symbol: GSTA2
• Gene ID (NCBI): 2939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Theranostics; Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening.; Authors - Zhenyu Wang
mouse	WB	J Invest Dermatol; Retracing from Outcomes to Causes: NRF2-Driven GSTA4 Transcriptional Regulation Controls Chronic Inflammation and Oxidative Stress in Atopic Dermatitis Recurrence; Authors - Xin Ma
rat	WB	Antioxidants (Basel); Isatin Counteracts Diethylnitrosamine/2-Acetylaminofluorene-Induced Hepatocarcinogenesis in Male Wistar Rats by Upregulating Anti-Inflammatory, Antioxidant, and Detoxification Pathways.; Authors - Nagwa G Tawfik
mouse	WB	Eur J Nutr; Multi-omics integration reveals the hepatoprotective mechanisms of ursolic acid intake against chronic alcohol consumption.; Authors - Xin Yan
human	WB	Kidney Blood Press Res; Identification of PDK4 as hub gene for diabetic nephropathy using co-expression network analysis; Authors - Yuanyuan Han
mouse	WB	J Pharmacol Sci; Enhanced expression of glutathione S-transferase in the hippocampus following acute treatment with trimethyltin in vivo.; Authors - Nagashima Reiko R

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iru (Verified Customer) (02-27-2019)

This antibody works best at 1:500 in TBST milk with O/N incubation. I get beautiful blot with the use of this antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Fallopian tube epithelial cells

GSTA2 Antibody Western Blot, validation (1:500 dilution) in Fallopian tube epithelial cells (Cat no:10505-1-AP)