GSDMB Polyklonaler Antikörper

GSDMB Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 12885-1-AP

Synonyme

gasdermin B, Gasdermin like protein, GSDMB, GSDML, PP4052, PRO2521

---

Galerie der Validierungsdaten

• 

  WB analysis of COLO 320 using 12885-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12885-1-AP (GSDMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12885-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12885-1-AP (GSDMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

12885-1-AP bindet in WB, IF, IHC, ELISA GSDMB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSDMB fusion protein Ag3895
• Vollständiger Name: gasdermin B
• Berechnetes Molekulargewicht: 411 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC025682
• Gene symbol: GSDMB
• Gene ID (NCBI): 55876
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC,WB	Front Immunol; Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma; Authors - Liangmin Fu ; KD Validated
human	WB, IHC	Int J Biol Sci; USP24-GSDMB complex promotes bladder cancer proliferation via activation of the STAT3 pathway.; Authors - Haiqing He
human	IHC	Front Oncol; Novel Pyroptosis-Related Gene Signatures Identified as the Prognostic Biomarkers for Bladder Carcinoma.; Authors - Jia You
human	WB	Toxicol Appl Pharmacol; Schisandrin B induces HepG2 cells pyroptosis by activating NK cells mediated anti-tumor immunity; Authors - Anping Song
human	IHC,IF	Cell Death Dis; Clinical-mediated discovery of pyroptosis in CD8+ T cell and NK cell reveals melanoma heterogeneity by single-cell and bulk sequence; Authors - Ying Zhang
human	WB	J Allergy Clin Immunol; A functional splice variant associated with decreased asthma risk abolishes the ability of gasdermin B to induce epithelial cell pyroptosis.; Authors - Ronald A Panganiban