GS28 Polyklonaler Antikörper

GS28 Polyklonal Antikörper für FC, IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16106-1-AP

Synonyme

28 kDa cis Golgi SNARE p28, 28 kDa Golgi SNARE protein, GOLIM2, GOS 28, GOS28, GOS28/P28, GOSR1, GS28, P28

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 16106-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16106-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16106-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 16106-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 16106-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16106-1-AP (GS28 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach cancer using 16106-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16106-1-AP (GS28 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 16106-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16106-1-AP (GS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 16106-1-AP

  1X10^6 HeLa cells were stained with 0.2ug GS28 antibody (16106-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HepG2-Zellen, humanes Lebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16106-1-AP bindet in WB, IHC, IF, FC,ELISA GS28 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GS28 fusion protein Ag9021
• Vollständiger Name: golgi SNAP receptor complex member 1
• Berechnetes Molekulargewicht: 28.6 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC012620
• Gene symbol: GOSR1
• Gene ID (NCBI): 9527
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)