GRWD1 Polyklonaler Antikörper

GRWD1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 10354-1-AP

Synonyme

GRWD, GRWD1, KIAA1942, RRB1, WDR28

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Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10354-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10354-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10354-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10354-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10354-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10354-1-AP (GRWD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 10354-1-AP

  IP result of anti-GRWD1 (IP:10354-1-AP, 4ug; Detection:10354-1-AP 1:1000) with PC-3 cells lysate 4000ug.

• 

  IHC staining of human prostate cancer using 10354-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10354-1-AP (GRWD1 antibody) at dilution of 1:100 (under 25x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, PC-3-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

10354-1-AP bindet in WB, IP, IHC, ELISA GRWD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRWD1 fusion protein Ag0367
• Vollständiger Name: glutamate-rich WD repeat containing 1
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC002440
• Gene symbol: GRWD1
• Gene ID (NCBI): 83743
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WD-repeat proteins are a class of functionally divergent molecules that cooperate with other proteins to regulate cellular processes. WD-repeat proteins have been implicated in a variety of cellular functions that are mostly regulatory in nature, including cytoskeleton organization, vesicular fusion, pre-mRNA splicing, and cell cycle regulation. WD-repeat-containing protein designated glutamate-rich WD repeat (GRWD1) is a component of the 50S and 80S preribosomal complexes and plays a role in ribosome biogenesis and during myeloid differentiation its levels are regulated. [PMID:15885502]

Publikationen

Species	Application	Title
human	WB,IHC	Exp Cell Res; GRWD1 promotes cell proliferation and migration in non-small cell lung cancer by activating the Notch pathway.; Authors - Qiongzi Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zehua (Verified Customer) (12-17-2019)

I identified siRNAs targeting GRWD1 with this antibody. It works well for WB. I strongly suggested it.

• Applications: Western Blot, Cell culture,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF-7

GRWD1 Antibody Western Blot,Cell culture, validation (1:1000 dilution) in MCF-7 (Cat no:10354-1-AP)