GRP94 Monoklonaler Antikörper

GRP94 Monoklonal Antikörper für FC (Intra), IF, WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

4G7C7

Kat-Nr. : CL594-60012

Synonyme

Cell and organelle markers, ECGP, endoplasmic reticulum marker, Endoplasmin, ER marker, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, Mitochondria Marker, Mitochondrial Marker, TRA1, Tumor rejection antigen 1

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Galerie der Validierungsdaten

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  WB analysis using CL594-60012

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-60012 (GRP94 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using CL594-60012

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated GRP94 antibody (CL594-60012, Clone: 4G7C7 ) at dilution of 1:100.

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  FC experiment of HeLa using CL594-60012

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GRP94 (CL594-60012, Clone:4G7C7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-60012 bindet in WB, IF, FC (Intra) GRP94 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GRP94 fusion protein Ag1439
• Vollständiger Name: heat shock protein 90kDa beta (Grp94), member 1
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC009195
• Gene symbol: HSP90B1
• Gene ID (NCBI): 7184
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C