GRIA1 Polyklonaler Antikörper

GRIA1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 25012-1-AP

Synonyme

GluA1, GLUH1, GluR 1, GluR A, GluR K1, GLUR1, GLURA, Glutamate receptor 1, GRIA1, HBGR1

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Galerie der Validierungsdaten

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  WB analysis using 25012-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25012-1-AP (GRIA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25012-1-AP (GRIA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 25012-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25012-1-AP (GRIA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 25012-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25012-1-AP (GRIA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25012-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25012-1-AP (GRIA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

25012-1-AP bindet in WB, IHC, ELISA GRIA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRIA1 fusion protein Ag21502
• Vollständiger Name: glutamate receptor, ionotropic, AMPA 1
• Berechnetes Molekulargewicht: 906 aa, 102 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC111734
• Gene symbol: GRIA1
• Gene ID (NCBI): 2890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors.

Publikationen

Species	Application	Title
mouse	WB	J Ethnopharmacol; Iridoids from Gardeniae fructus ameliorates depression by enhancing synaptic plasticity via AMPA receptor-mTOR signaling; Authors - Baomei Xia