GREM2 Polyklonaler Antikörper

GREM2 Polyklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13892-1-AP

Synonyme

Gremlin2, Gremlin 2, GREM 2, DAND3, DAND 3

---

Galerie der Validierungsdaten

• 

  IHC staining of mouse ovary using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13892-1-AP (GREM2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13892-1-AP (GREM2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13892-1-AP (GREM2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13892-1-AP (GREM2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse kidney using 13892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using GREM2 antibody (13892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse ovary using 13892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using GREM2 antibody (13892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maus-Eierstockgewebe, humanes Eierstockgewebe, humanes Nierengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe, Maus-Eierstockgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

13892-1-AP bindet in WB, IHC, IF-P, ELISA GREM2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GREM2 fusion protein Ag4854
• Vollständiger Name: gremlin 2, cysteine knot superfamily, homolog (Xenopus laevis)
• Berechnetes Molekulargewicht: 168 aa, 19 kDa
• GenBank-Zugangsnummer: BC046632
• Gene symbol: GREM2
• Gene ID (NCBI): 64388
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IHC	Am J Pathol; The Onset of Heat-Induced Testicular Calcification in Mice: Involvement of the Telomeric Locus on Chromosome 1.; Authors - Masataka Chihara
human	IF	Stem Cells Dev; Coordinated proliferation and differentiation of human iPS cell-derived cardiac progenitor cells depends on BMP signaling regulation by Gremlin 2.; Authors - Jeffery Bylund
mouse	IHC	Andrology; Genetic factors derived from the MRL/MpJ mouse function to maintain the integrity of spermatogenesis after heat exposure.; Authors - M Chihara
mouse	WB,IHC	Metabolism; SGLT2 inhibitor empagliflozin downregulates miRNA-34a-5p and targets GREM2 to inactivate hepatic stellate cells and ameliorate non-alcoholic fatty liver disease-associated fibrosis; Authors - Yunfeng Shen