GPX7 Polyklonaler Antikörper

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• KD/KO Validated

GPX7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 13501-1-AP

Synonyme

CL683, glutathione peroxidase 7, GPx 7, GPX6, GPX7, GSHPx 7

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Galerie der Validierungsdaten

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  WB analysis using 13501-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13501-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13501-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 13501-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13501-1-AP (GPX7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13501-1-AP (GPX7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 13501-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, humanes Hirngewebe, humanes Plazenta-Gewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

13501-1-AP bindet in WB, IF, IHC, ELISA GPX7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPX7 fusion protein Ag4328
• Vollständiger Name: glutathione peroxidase 7
• Berechnetes Molekulargewicht: 187 aa, 21 kDa
• Beobachtetes Molekulargewicht: 18-21 kDa
• GenBank-Zugangsnummer: BC032788
• Gene symbol: GPX7
• Gene ID (NCBI): 2882
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPX7(Glutathione peroxidase 7) is also named as GPX6,CL683,GSHPx-7 and belongs to the glutathione peroxidase family.It shares approximately 45% amino acid sequence with other GPX family members.Because GPX7 expression protected cells from acidic bile acidinduced DNA damage, it may protect oesophageal epithelial cells from acidic bile acid-induced apoptosis(PMID:22157330). This antibody can ve detected as 18-21kDa due to signal peptide splicing.

Publikationen

Species	Application	Title
human	WB	Gut; Glutathione peroxidase 7 protects against oxidative DNA damage in oesophageal cells.; Authors - Peng Dunfa D
human	WB	Gut; Glutathione peroxidase 7 has potential tumour suppressor functions that are silenced by location-specific methylation in oesophageal adenocarcinoma.; Authors - Peng Dunfa D
mouse	WB	EMBO Mol Med; Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human.; Authors - Chang Yi-Cheng YC
human	IHC	Oncogene; Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.; Authors - Qingcai Meng
human	WB	Carcinogenesis; Loss of glutathione peroxidase 7 promotes TNF-α-induced NF-κB activation in Barrett's carcinogenesi.; Authors - Dun-Fa Peng ; KD Validated
	WB	Free Radic Biol Med; GPx8 peroxidase prevents leakage of H2O2 from the endoplasmic reticulum.; Authors - Thomas Ramming