GPT2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GPT2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC (Intra), FC, ELISA

Konjugation

Unkonjugiert

Publikationen

29

Kat-Nr. : 16757-1-AP

Synonyme

AAT2, Alanine aminotransferase 2, ALAT2, ALT2, GPT 2, GPT2

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Galerie der Validierungsdaten

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  WB analysis using 16757-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16757-1-AP (GPT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16757-1-AP

  WB result of GPT2 antibody (16757-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPT2 transfected HepG2 cells.

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  WB analysis of mouse kidney using 16757-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16757-1-AP (GPT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 16757-1-AP

  IP Result of anti-GPT2 (IP:16757-1-AP, 4ug; Detection:16757-1-AP 1:500) with mouse kidney tissue lysate 7200ug.

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  IHC staining of human skeletal muscle using 16757-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16757-1-AP (GPT2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 16757-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16757-1-AP (GPT2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 16757-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16757-1-AP (GPT2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16757-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16757-1-AP (GPT2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16757-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT2 antibody (16757-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 16757-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human GPT2 (16757-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, Mausnierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 24 publications below
• IHC: See 7 publications below
• IF: See 4 publications below

Produktinformation

16757-1-AP bindet in WB, IP, IHC, IF, FC (Intra), FC, ELISA GPT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPT2 fusion protein Ag10273
• Vollständiger Name: glutamic pyruvate transaminase (alanine aminotransferase) 2
• Berechnetes Molekulargewicht: 523 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa, 47 kDa
• GenBank-Zugangsnummer: BC062555
• Gene symbol: GPT2
• Gene ID (NCBI): 84706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPT2 (Alanine aminotransferase 2; ALT2) is a key enzyme in intermediatary metabolism, catalysing the reversible transfer of the amino group of glutamate to pyruvate, yielding alanine and ketoglutarate. GPT2 protein localizes to mitochondria, and is mainly expressed in the pancreas, brain, adrenal gland, skeletal muscle and heart. Serum GPT2 is a useful marker for liver damage. Recently mutation of GPT2 has been reported to be a cause of neural disorder (27601654). 16757-1-AP antibody recognizes two different isoforms of GPT2 protein.

Publikationen

Species	Application	Title
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
mouse	WB	Cell Res; Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.; Authors - Wei-Feng Cai ; KD Validated
human	WB	Nat Commun; Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.; Authors - Yun-Qian Wang
human	WB	Cancer Res; Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.; Authors - Bo Zhang
MOUSE	WB,IF	Proc Natl Acad Sci U S A; Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features.; Authors - Qing Ouyang
human	IHC	Cell Rep; Thyroid hormone regulates glutamine metabolism and anaplerotic fluxes by inducing mitochondrial glutamate aminotransferase GPT2; Authors - Annunziata Gaetana Cicatiello

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