GPSM1 Polyklonaler Antikörper

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• KD/KO Validated

GPSM1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11483-1-AP

Synonyme

G-protein-signaling modulator 1, AGS3, Activator of G-protein signaling 3

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11483-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11483-1-AP (AGS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11483-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11483-1-AP (AGS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11483-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11483-1-AP (AGS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11483-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11483-1-AP (AGS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11483-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11483-1-AP (AGS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus cancer using 11483-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11483-1-AP (GPSM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 11483-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11483-1-AP (GPSM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11483-1-AP

  Immunofluorescent analysis of HepG2 cells, using GPSM1 antibody 11483-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, Maushirngewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

11483-1-AP bindet in WB, IHC, IF/ICC, ELISA GPSM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPSM1 fusion protein Ag2018
• Vollständiger Name: G-protein signaling modulator 1 (AGS3-like, C. elegans)
• Berechnetes Molekulargewicht: 652 aa, 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC017353
• Gene symbol: GPSM1
• Gene ID (NCBI): 26086
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPSM1, also named as AGS3, is a receptor-independent G protein activator that has been implicated in multiple biological events such as brain development, neuroplasticity and addiction, cardiac function, Golgi structure/function, macroautophagy and metabolism. It contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. It has been shown that AGS3 could regulate the orientation of the mitotic spindle, cAMP production, membrane protein transport, and asymmetric cell division by binding preferentially to inactive Gai/o subunits complexed with guanine dinucleotide phosphate (GDP) at multiple G-protein regulatory or GoLoco motif repeats. It also plays an important anti-apoptotic role through enhancing the phosphorylation of cyclic AMP response element-binding protein (p-CREB).

Publikationen

Species	Application	Title
mouse	WB,IHC,IF	Nat Commun; GPSM1 impairs metabolic homeostasis by controlling a pro-inflammatory pathway in macrophages; Authors - Jing Yan
rat	WB,IHC	J Ovarian Res; Depletion of GPSM1 enhances ovarian granulosa cell apoptosis via cAMP-PKA-CREB pathway in vitro.; Authors - Xuzi Cai ; KD Validated
mouse	WB,IF	Mol Metab; GPSM1 in POMC neurons impairs brown adipose tissue thermogenesis and provokes diet-induced obesity; Authors - Mengyang Tang