GPR137 Polyklonaler Antikörper

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• KD/KO Validated

GPR137 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11929-1-AP

Synonyme

C11orf4, G protein coupled receptor 137, GPR137, GPR137A, TM7SF1L1

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Galerie der Validierungsdaten

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  IHC staining of mouse brain using 11929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11929-1-AP (GPR137 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11929-1-AP (GPR137 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11929-1-AP (GPR137 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11929-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11929-1-AP (GPR137 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11929-1-AP bindet in WB, IF, IHC, ELISA GPR137 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPR137 fusion protein Ag2611
• Vollständiger Name: G protein-coupled receptor 137
• Berechnetes Molekulargewicht: 396 aa, 44 kDa
• GenBank-Zugangsnummer: BC033920
• Gene symbol: GPR137
• Gene ID (NCBI): 56834
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

G protein-coupled receptors (GPRs) are highly related to oncogenesis and cancer metastasis. G protein-coupled receptor 137 (GPR137) was initially reported as a novel orphan GPR about 10 years ago. GPR137 is ubiquitously expressed in the central nervous system (CNS), mainly in the hippocampus (PMID: 22025619). GPR137 is involved in the progression of human glioma, suggesting GPR137 as a potential oncogene of glioma cells (PMID: 24870220). Recent study shows that GPR137 plays an important role in colon cancer cell proliferation (PMID: 25301753).

Publikationen

Species	Application	Title
human	WB	Biotechnol Appl Biochem; Knockdown of GPR137 by RNAi inhibits pancreatic cancer cell growth and induces apoptosis.; Authors - Xianping Cui
human	WB, IF	Biotechnol Appl Biochem; G-protein-coupled receptor 137 accelerates proliferation of urinary bladder cancer cells in vitro.; Authors - Yiheng Du ; KD Validated
human	WB	Cell Biol Int; Down-Regulation of G Protein-Coupled Receptor 137 by RNA Interference Inhibits Cell Growth of Two Hepatoma Cell Lines.; Authors - Xin Shao
human	IHC	Neurol Sci; Inhibition of GPR137 expression reduces the proliferation and colony formation of malignant glioma cells.; Authors - Gang Zong
human	WB	J Bone Oncol; Small interfering RNA-mediated silencing of G-protein-coupled receptor 137 inhibits growth of osteosarcoma cells.; Authors - Hao Li
human	WB	Mol Med Rep; RNA interference-mediated silencing of G protein-coupled receptor 137 inhibits human gastric cancer cell growth.; Authors - Zishu Wang ; KD Validated