golgin 97 Polyklonaler Antikörper

golgin 97 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Rezensionen (2)

Kat-Nr. : 12640-1-AP

Synonyme

GOLGA1, Golgin-97, Golgin subfamily A member 1

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Galerie der Validierungsdaten

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  WB analysis of SMMC-7721 using 12640-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12640-1-AP (golgin 97 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12640-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12640-1-AP (golgin 97 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12640-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12640-1-AP (golgin 97 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 12640-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (12640-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12640-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (12640-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 12640-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human golgin 97 (12640-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, SMMC-7721-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 10 publications below
• IP: See 1 publications below

Produktinformation

12640-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA golgin 97 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: golgin 97 fusion protein Ag3335
• Vollständiger Name: golgi autoantigen, golgin subfamily a, 1
• Berechnetes Molekulargewicht: 767 aa, 88 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: BC032853
• Gene symbol: golgin 97
• Gene ID (NCBI): 2800
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa, and Catalog#12640-1-AP specially recognises the 97 kDa protein. IF result shows strong Gogli staining.

Publikationen

Species	Application	Title
	IF,WB	Nat Commun; TBC1D23 mediates Golgi-specific LKB1 signaling; Authors - Yingfeng Tu
mouse	IF	Nat Commun; Depletion of mitochondrial methionine adenosyltransferase α1 triggers mitochondrial dysfunction in alcohol-associated liver disease.; Authors - Lucía Barbier-Torres
human,mouse	IF	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
human	WB	Dev Cell; GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion.; Authors - Xiaoyan Zhang
human	WB	Cell Rep; CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth; Authors - Shasha Yin
human	IF	Cell Chem Biol; Artemisinin inhibits NRas palmitoylation by targeting the protein acyltransferase ZDHHC6.; Authors - Nan Qiu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (04-17-2023)

very good for WB

• Applications: Western Blot

FH

Boyan (Verified Customer) (04-02-2023)

very good for WB.

• Applications: Western Blot