GNB1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GNB1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 10247-2-AP

Synonyme

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, GBB1

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Galerie der Validierungsdaten

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  WB analysis using 10247-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10247-2-AP (GNB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10247-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10247-2-AP (GNB1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 10247-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10247-2-AP (GNB1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human gliomas using 10247-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10247-2-AP (GNB1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 10247-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GNB1 antibody (10247-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 3 publications below

Produktinformation

10247-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA GNB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNB1 fusion protein Ag0313
• Vollständiger Name: guanine nucleotide binding protein (G protein), beta polypeptide 1
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC004186
• Gene symbol: GNB1
• Gene ID (NCBI): 2782
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNB1(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285).

Publikationen

Species	Application	Title
mouse	WB,IF,IP	Cell; Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.; Authors - Gonzalo P Solis ; KD Validated
human	IF,IP	PLoS Pathog; H9N2 virus-derived M1 protein promotes H5N6 virus release in mammalian cells: Mechanism of avian influenza virus inter-species infection in humans.; Authors - Fangtao Li
mouse	WB	Neuropharmacology.; A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission.; Authors - Saba LM
mouse	IHC	Exp Eye Res; Nr2e3-directed transcriptional regulation of genes involved in photoreceptor development and cell-type specific phototransduction.; Authors - Haider Neena B NB
mouse	WB	Dev Dyn; Neural tube defects and impaired neural progenitor cell proliferation in Gbeta1-deficient mice.; Authors - Okae Hiroaki H
human	WB	Bioengineered; Shikonin induces cell autophagy via modulating the microRNA -545-3p/guanine nucleotide binding protein beta polypeptide 1 axis, thereby disrupting cellular carcinogenesis in colon cancer.; Authors - ZhiWei Hu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nick (Verified Customer) (04-20-2022)

If I could give this three thumbs up, I would.

• Applications: Western Blot