GNAI3 Polyklonaler Antikörper

GNAI3 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11641-1-AP

Synonyme

Guanine nucleotide-binding protein G(i) subunit alpha-3, G(i) alpha-3, 87U6

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Galerie der Validierungsdaten

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  WB analysis using 11641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11641-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11641-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11641-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11641-1-AP (GNAI3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 11641-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11641-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 11641-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 11641-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11641-1-AP (GNAI3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 11641-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GNAI3 antibody (11641-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of A431 using 11641-1-AP

  1X10^6 A431 cells were stained with .2ug GNAI3 antibody (11641-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattennierengewebe, A431-Zellen, humanes Nierengewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

11641-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA GNAI3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNAI3 fusion protein Ag2229
• Vollständiger Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: ~40 kDa
• GenBank-Zugangsnummer: BC025285
• Gene symbol: GNAI3
• Gene ID (NCBI): 2773
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNAI3 is a member of the Gi group proteins, which belong to the Gα protein family. GNAI3 is involved in several critical biological processes and regulates many cellular activities, including proliferation, differentiation, apoptosis, and migration. For example, GNAI3 can regulate a class of K+ channels in response to hormone and neurotransmitter signals. In addition to its function as a downstream signal pathway switch for receptors on the plasma membrane, GNAI3 has also been shown to localize to the centrosome and affect cytokinesis. These indicate that GNAI3 has important receptor-independent functions. The molecular weight of GNAI3 is 40 kDa. (PMID: 25444921, 34803495)

Publikationen

Species	Application	Title
human	WB	Sci Signal; Growth factor-dependent phosphorylation of Gαi shapes canonical signaling by G protein-coupled receptors; Authors - Suchismita Roy
mouse	WB	Cell Signal; RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.; Authors - Menbere Wendimu