Glutamine Synthetase Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Glutamine Synthetase Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

31

Rezensionen (3)

Kat-Nr. : 11037-2-AP

Synonyme

GLUL, GS, Glutamate--ammonia ligase, GLNS, EC:6.3.1.2

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Galerie der Validierungsdaten

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  WB analysis using 11037-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11037-2-AP

  WB result of Glutamine synthetase antibody (11037-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells.

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  IHC staining of mouse liver using 11037-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11037-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 11037-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 11037-2-AP (Glutamine synthetase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse liver using 11037-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Glutamine Synthetase antibody (11037-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human brain using 11037-2-AP

  Immunofluorescent analysis of (4% PFA) fixed human brain tissue using 11037-2-AP (Glutamine synthetase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 11037-2-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug Glutamine Synthetase Polyclonal antibody (11037-2-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe, humanes Hirngewebe, Maushirngewebe
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Produktinformation

11037-2-AP bindet in WB, IHC, IF-P, FC (Intra), IP, ELISA Glutamine Synthetase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Glutamine Synthetase fusion protein Ag1510
• Vollständiger Name: glutamate-ammonia ligase (glutamine synthetase)
• Berechnetes Molekulargewicht: 374 aa, 42 kDa
• Beobachtetes Molekulargewicht: 40-42 kDa
• GenBank-Zugangsnummer: BC011700
• Gene symbol: Glutamine Synthetase
• Gene ID (NCBI): 2752
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).

Publikationen

Species	Application	Title
mouse	IF	Nat Methods; Spatially resolved isotope tracing reveals tissue metabolic activity.; Authors - Lin Wang
mouse	WB,IHC	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou ; KD Validated
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
human,mouse	IF,WB	Protein Cell; Follicle stimulating hormone controls granulosa cell glutamine synthesis to regulate ovulation; Authors - Kai-Hui Zhang
human	WB	Sci Transl Med; An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs.; Authors - Wei-Jian Li
mouse	WB	Cell Res; Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.; Authors - Wei-Feng Cai ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrea (Verified Customer) (09-06-2023)

antibody works perfect

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse retina

FH

Melanie (Verified Customer) (02-02-2023)

Good antibody, which works well in HEK293T cells

• Applications: Western Blot
• Cell Tissue Type: HEK293T cells

FH

Ryan (Verified Customer) (02-27-2019)

Tris-HCL antigen retrieval ph=9.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish retina (Muller glia) at 3dpf

Glutamine Synthetase Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish retina (Muller glia) at 3dpf (Cat no:11037-2-AP)