KGA/GAM/GAC Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KGA/GAM/GAC Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 23549-1-AP

Synonyme

AAD20, GAC, GAM, GLS, GLS1, glutaminase, K glutaminase, KGA, KGA/GAM/GAC, KIAA0838, L glutamine amidohydrolase

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 23549-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 23549-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 23549-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 23549-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23549-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 23549-1-AP

  IP Result of anti-KGA/GAM/GAC (IP:23549-1-AP, 4ug; Detection:23549-1-AP 1:1000) with HEK-293 cells lysate 1200ug.

• 

  IHC staining of human kidney using 23549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 23549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

• 

  IHC staining of human brain using 23549-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

• 

  IHC staining of human brain using 23549-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

• 

  IF Staining of HEK-293 using 23549-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 23549-1-AP (KGA/GAM/GAC antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 23549-1-AP

  Immunofluorescent analysis of HEK-293 cells using 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HeLa using 23549-1-AP

  Immunofluorescent analysis of HeLa cells using 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Mauslebergewebe, Rattenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:25-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

23549-1-AP bindet in WB, IP, IHC, IF, ELISA KGA/GAM/GAC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KGA/GAM/GAC fusion protein Ag20354
• Vollständiger Name: glutaminase
• Berechnetes Molekulargewicht: 669 aa, 73 kDa
• Beobachtetes Molekulargewicht: 58 kDa, 65 kDa, 83 kDa
• GenBank-Zugangsnummer: BC038507
• Gene symbol: GLS
• Gene ID (NCBI): 2744
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa(PMID:12408749). Ping Gao et al. (2009) determined that mitochondrial glutaminase expression (GLS, molecular mass of ~58 kDa) is increased ~10-fold in response to Myc. It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). GLS has 3 isoforms produced by alternative splicing and this antibody can recognize all the 3 isoforms(KGA,GAM,GAC) of GLS.

Publikationen

Species	Application	Title
human	WB,IF	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KO Validated
human	WB	Redox Biol; Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels.; Authors - M Ryan Smith