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GLRA3 Polyklonaler Antikörper

GLRA3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13145-1-AP

Synonyme

GLRA3, glycine receptor, alpha 3

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of MCF-7 cells using GLRA3 Polyclonal antibody (13145-1-AP)

    WB analysis of MCF-7 using 13145-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13145-1-AP (GLRA3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMCF7 cells

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 2 publications below

Produktinformation

13145-1-AP bindet in WB, ELISA GLRA3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GLRA3 fusion protein Ag3702
Vollständiger Name glycine receptor, alpha 3
Berechnetes Molekulargewicht 464 aa, 54 kDa
Beobachtetes Molekulargewicht 54 kDa
GenBank-ZugangsnummerBC036086
Gene symbol GLRA3
Gene ID (NCBI) 8001
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Protokolle

Produktspezifische Protokolle
WB protocol for GLRA3 antibody 13145-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

PLoS Biol

mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.

Authors - Zi-Yang Zhang
View Article
humanWB

Neuropharmacology

Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.

Authors - Zi-Yang Zhang
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Braulio (Verified Customer) (02-26-2020)

Western blot in lumbar spinal cord samples, shows a mild band for alpha 3

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Spinal cord