GLRA2 Polyklonaler Antikörper

GLRA2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 13831-1-AP

Synonyme

GLR, GLRA2, glycine receptor, alpha 2

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Galerie der Validierungsdaten

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  WB analysis of PBMC cells using 13831-1-AP

  WB result of GLRA2 antibody (13831-1-AP, 1:600) with PBMC cells. Proteintech αGLRA2 13831-1-AP in 10% BSA / TBS 0.05% Tween-20 O/N @ 4°C Goat anti-rabbit HRP 1:10000 in 5% milk / TBS 0.05% Tween-20 1h @ RT.

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  WB analysis using 13831-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13831-1-AP (GLRA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13831-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13831-1-AP (GLRA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 13831-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13831-1-AP (GLRA2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PBMC-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13831-1-AP bindet in WB, IHC, ELISA GLRA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLRA2 fusion protein Ag4743
• Vollständiger Name: glycine receptor, alpha 2
• Berechnetes Molekulargewicht: 452 aa, 52 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC032864
• Gene symbol: GLRA2
• Gene ID (NCBI): 2742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chelsea (Verified Customer) (02-17-2023)

We transfected HEK Cells with Glycine Receptor Alpha 1-2-3 to validate the GlyRa2 antibody. However, we saw staining in all conditions, even non transfected HEK cells (which have been validated to not contain endogenous GlyRa2). Therefore, we assume that the GlyRa2 antibody is not specific and binds an aspecifiek endogenous molecule.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Transfected HEK Cells