GHRL Polyklonaler Antikörper

GHRL Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13309-1-AP

Synonyme

Appetite regulating hormone, Ghrelin Obestatin, GHRL, Motilin related peptide, MTLRP, obestatin, Protein M46

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Galerie der Validierungsdaten

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  WB analysis of human stomach using 13309-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13309-1-AP (GHRL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 13309-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 13309-1-AP (GHRL Antibody) at dilution of 1:400.

• 

  IHC staining of human stomach using 13309-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 13309-1-AP (GHRL Antibody) at dilution of 1:400.

• 

  IHC staining of rat stomach using 13309-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach slide using 13309-1-AP (GHRL Antibody) at dilution of 1:50.

• 

  IHC staining of rat stomach using 13309-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach slide using 13309-1-AP (GHRL Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Magengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe, Ratten-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

13309-1-AP bindet in WB, IHC, ELISA GHRL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GHRL fusion protein Ag4141
• Vollständiger Name: ghrelin/obestatin prepropeptide
• Berechnetes Molekulargewicht: 117 aa, 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC025791
• Gene symbol: GHRL
• Gene ID (NCBI): 51738
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GHRL encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is predominantly produced in endocrine cells in the gastric mucosa, called X/A-like or ghrelin cells, from where it is secreted into the plasma. In the pituitary gland, ghrelin stimulates GH release and regulates food intake and energy metabolism. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes.

Publikationen

Species	Application	Title
rat	WB	Phytomedicine; Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.; Authors - Ping Ma
rat	WB	Pancreas; Effects of Ghrelin miRNA on Inflammation and Calcium Pathway in Pancreatic Acinar Cells of Acute Pancreatitis; Authors -
human	IHC	Biosci Rep; Differential expression of ghrelin and GHSR via the mTOR pathway during the dynamic carcinogenic process involving oral, potentially malignant disorders.; Authors - Jianing Lou
mouse	IHC	Neuroreport; Expression of ghrelin or growth hormone secretagogue receptor in the brain of postpartum stress mice.; Authors - Jing-Wei Xing
rat	WB	Hepatobiliary Pancreat Dis Int; Ghrelin inhibits IKKβ/NF-κB activation and reduces pro-inflammatory cytokine production in pancreatic acinar AR42J cells treated with cerulein.; Authors - Ren-Jie Chang