GGH Polyklonaler Antikörper

GGH Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13264-1-AP

Synonyme

Conjugase, Gamma Glu X carboxypeptidase, Gamma glutamyl hydrolase, GGH, GH

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Galerie der Validierungsdaten

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  WB analysis using 13264-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13264-1-AP (GGH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13264-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13264-1-AP (GGH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13264-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13264-1-AP (GGH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13264-1-AP

  IP result of anti-GGH (IP:13264-1-AP, 4ug; Detection:13264-1-AP 1:500) with HepG2 cells lysate 5600 ug.

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  IHC staining of human kidney using 13264-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13264-1-AP (GGH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13264-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13264-1-AP (GGH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HepG2-Zellen, HT-1080.Zellen, MCF-7-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13264-1-AP bindet in WB, IP, IF, IHC, ELISA GGH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GGH fusion protein Ag3939
• Vollständiger Name: gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
• Berechnetes Molekulargewicht: 318 aa, 36 kDa
• Beobachtetes Molekulargewicht: 30-37 kDa, 55 kDa
• GenBank-Zugangsnummer: BC025025
• Gene symbol: GGH
• Gene ID (NCBI): 8836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GGH (Gamma glutamyl hydrolase), also named as GH or Conjugase, is a key lysosomal enzyme involved in the metabolism of folic acid and in the action of antifolate drugs (PMID: 16945597). GGH catalyzes the removal of γ-linked polyglutamates from the intracellular folylpolyglutamates to yield folylmonoglutamate coenzymes (PMID: 9614206). The full-length protein has a calculated molecular mass of 36 kDa, contains four potential asparagine glycosylation sites, and was predicted to have a 24-amino-acid signal peptide (PMID: 8816764). GGH can form homodimer which contains two potential active sites (PMID: 16945597). Some bands can be detected by SDS-PAGE: 35-37kDa (full-length), 55kDa (glycosylated form) and 30-33kDa (signal peptide removed) (PMID: 8621474).

Publikationen

Species	Application	Title
	WB	Front Pharmacol; Up-regulation of ABCG1 is associated with methotrexate resistance in acute lymphoblastic leukemia cells; Authors - Yao Chen
human	IHC,IF	Reprod Sci; The Identification of Gamma-Glutamyl Hydrolase in Uterine Corpus Endometrial Carcinoma: a Predictive Model and Machine Learning; Authors - Cheng Zhu