BMP9 Polyklonaler Antikörper

BMP9 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17769-1-AP

Synonyme

BMP 9, BMP9, BMP-9, Bone morphogenetic protein 9, GDF 2, GDF2

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Galerie der Validierungsdaten

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  WB analysis of L02 using 17769-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17769-1-AP (BMP9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 17769-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 17769-1-AP (BMP9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17769-1-AP bindet in WB, IHC,ELISA BMP9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BMP9 fusion protein Ag12177
• Vollständiger Name: growth differentiation factor 2
• Berechnetes Molekulargewicht: 429 aa, 47 kDa
• Beobachtetes Molekulargewicht: 30 kDa, 46 kDa
• GenBank-Zugangsnummer: BC074921
• Gene symbol: GDF2
• Gene ID (NCBI): 2658
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BMP9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia. Human BMP9 precursor protein contains 429 amino acids and has a calculated molecular mass of about 47 kD. Posttranslational processing results in a mature BMP9 protein of about 13 kD. Once secreted, BMP9 may exist as dimers of mature regions or as BMP9 proregion complexes in which the proregion of BMP9 remains tightly associated with the mature dimer.