GDAP1 Polyklonaler Antikörper

GDAP1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13152-1-AP

Synonyme

CMTRIA, GDAP1

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Galerie der Validierungsdaten

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  WB analysis using 13152-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 13152-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of mouse brain using 13152-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of human brain using 13152-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, fetales humanes Hirngewebe, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13152-1-AP bindet in WB, IHC, ELISA GDAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GDAP1 fusion protein Ag3769
• Vollständiger Name: ganglioside-induced differentiation-associated protein 1
• Berechnetes Molekulargewicht: 358 aa, 41 kDa
• Beobachtetes Molekulargewicht: 36-41 kDa
• GenBank-Zugangsnummer: BC024939
• Gene symbol: GDAP1
• Gene ID (NCBI): 54332
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GDAP1 is a member of the ganglioside-induced differentiation-associated protein family, which may play a role in a signal transduction pathway during neuronal development. Mutations in this gene have been associated with various forms of Charcot-Marie-Tooth Disease and neuropathy.