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  • Featured Product
  • KD/KO Validated

GBP5 Polyklonaler Antikörper

GBP5 Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-13220

Synonyme

GBP 5, GBP TA antigen, GBP5, GTP binding protein 5, guanylate binding protein 5

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated GBP5 Polyclonal anti (CL488-13220)

    FC experiment of MCF-7 using CL488-13220

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GBP5 (CL488-13220) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-13220 bindet in FC (Intra) GBP5 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GBP5 fusion protein Ag4103
Vollständiger Name guanylate binding protein 5
Berechnetes Molekulargewicht 586 aa, 67 kDa
Beobachtetes Molekulargewicht 65 kDa, 55 kDa
GenBank-ZugangsnummerBC031639
Gene symbol GBP5
Gene ID (NCBI) 115362
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 488 GBP5 antibody CL488-13220Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen