GATA2 Polyklonaler Antikörper

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• KD/KO Validated

GATA2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 11103-1-AP

Synonyme

NFE1B, GATA-binding protein 2, GATA binding protein 2, Endothelial transcription factor GATA-2

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Galerie der Validierungsdaten

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  WB analysis using 11103-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11103-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11103-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11103-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 11103-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11103-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11103-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11103-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11103-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11103-1-AP (GATA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 11103-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11103-1-AP (GATA2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of SH-SY5Y using 11103-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GATA2 antibody (11103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, K-562-Zellen, Mausherzgewebe, Mauslebergewebe, Rattenherzgewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• ChIP: See 2 publications below

Produktinformation

11103-1-AP bindet in WB, IHC, IF/ICC, ChIP, ELISA GATA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GATA2 fusion protein Ag1557
• Vollständiger Name: GATA binding protein 2
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC018988
• Gene symbol: GATA2
• Gene ID (NCBI): 2624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the molecular weight of GATA2? Is GATA2 post-translationally modified?

The molecular weight of GATA2 is 51 kDa. There are two known isoforms of GATA2 that use alternative first exons (PMID: 10731672). GATA2 can be phosphorylated, which regulates its activity (PMID: 7876160).

 What is the subcellular localization of GATA2?

GATA2 acts as a transcription factor in the nucleus. However, it can also be found in the cytoplasm (PMID: 25163535) and its phosphorylation promotes nuclear localization (PMID: 15837948).

 What is the tissue expression pattern of GATA2?

GATA2 was initially identified as a hematopoietic transcription factor regulating the differentiation of hematopoietic stem cells but is also present in early erythroid cells, mast cells, and megakaryocytes. Additionally, it is expressed in the central nervous system (PMID: 10357893).

Publikationen

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IF	EMBO J; Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells.; Authors - Yanni Ma
human	WB	BMC Med; Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis; Authors - Zhijian Zheng
human	WB	PLoS Genet; The PU.1-Modulated MicroRNA-22 Is a Regulator of Monocyte/Macrophage Differentiation and Acute Myeloid Leukemia.; Authors - Chao Shen
human	WB	Int J Mol Sci; LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases.; Authors - Manabu Ogoyama
human	ChIP,IF	Endocrinology; PGE2 and Thrombin Induce Myofibroblast Transdifferentiation via Activin A and CTGF in Endometrial Stromal Cells.; Authors - Kazuya Kusama