GATA1 Monoklonaler Antikörper

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• KD/KO Validated

GATA1 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

5E2A8

Kat-Nr. : CL555-60011

Synonyme

5E2A8, ERYF1, GATA-1, GATA-binding factor 1, GF1

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  IF Staining of HepG2 using CL555-60011

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 GATA1 antibody (CL555-60011, Clone: 5E2A8 ) at dilution of 1:200.

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  FC experiment of Jurkat using CL555-60011

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human GATA1 (CL555-60011, Clone:5E2A8) (red), or 0.4 ug CoraLite®555 Mouse IgG1 Isotype Control (MOPC-21) (CL555-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-60011 bindet in IF/ICC, FC (Intra) GATA1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GATA1 fusion protein Ag1350
• Vollständiger Name: GATA binding protein 1 (globin transcription factor 1)
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC009797
• Gene symbol: GATA1
• Gene ID (NCBI): 2623
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].