GAPDHS Polyklonaler Antikörper

GAPDHS Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 13937-1-AP

Synonyme

GAPD2, GAPDH 2, GAPDH2, GAPDHS, GAPDS, HSD 35

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Galerie der Validierungsdaten

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  WB analysis using 13937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13937-1-AP (GAPDHS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13937-1-AP (GAPDHS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below

Produktinformation

13937-1-AP bindet in WB, ELISA GAPDHS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GAPDHS fusion protein Ag4988
• Vollständiger Name: glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC036373
• Gene symbol: GAPDHS
• Gene ID (NCBI): 26330
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAPDHS(Glyceraldehyde-3-phosphate dehydrogenase, testis-specific) is also named as GAPD2, GAPDH2, GAPDS and belongs to the glyceraldehyde-3-phosphate dehydrogenase family. It may play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. GAPDHS is a promising contraceptive target because it is specific to male germ cells, essential for sperm motility and male fertility, and well suited to pharmacological inhibition(PMID:20828617).

Publikationen

Species	Application	Title
mouse	WB	J Hazard Mater; Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.; Authors - Zijun Peng
human	WB	Theranostics; Phosphorylation of IRS4 by CK1γ2 promotes its degradation by CHIP through the ubiquitin/lysosome pathway.; Authors - Xinchun Li
human	WB	Theranostics; Up-regulation of PCOLCE by TWIST1 promotes metastasis in Osteosarcoma.; Authors - Shang Wang
mouse	WB	Oxid Med Cell Longev; Withaferin A Exerts Preventive Effect on Liver Fibrosis through Oxidative Stress Inhibition in a Sirtuin 3-Dependent Manner.; Authors - Jingya Gu
rat	WB	J Cell Mol Med; Celastrol exerts anti-inflammatory effect in liver fibrosis via activation of AMPK-SIRT3 signalling.; Authors - Yuqin Wang
human	WB	Front Cell Dev Biol; Sperm-Specific Glycolysis Enzyme Glyceraldehyde-3-Phosphate Dehydrogenase Regulated by Transcription Factor SOX10 to Promote Uveal Melanoma Tumorigenesis.; Authors - Xia Ding