GAPDH Monoklonaler Antikörper

GAPDH Monoklonal Antikörper für FC (Intra), WB

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hefe, human, Maus, Pflanze, Ratte, Zebrafisch

Anwendung

WB, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen

3

CloneNo.

1E6D9

Kat-Nr. : CL594-60004

Synonyme

G3PD, GAPD, GAPDH, OK/SW cl.12

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Galerie der Validierungsdaten

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  WB analysis using CL594-60004

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-60004 (GAPDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using CL594-60004

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human GAPDH (CL594-60004, Clone:1E6D9) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

CL594-60004 bindet in WB, FC (Intra) GAPDH und zeigt Reaktivität mit Hefe, human, Maus, Pflanze, Ratten, Zebrafisch

• Getestete Reaktivität: Hefe, human, Maus, Pflanze, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GAPDH fusion protein Ag0766
• Vollständiger Name: glyceraldehyde-3-phosphate dehydrogenase
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC004109
• Gene symbol: GAPDH
• Gene ID (NCBI): 2597
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Publikationen

Species	Application	Title
mouse	WB	Brain Sci; Itaconate Attenuates Neuroinflammation and Exerts Dopamine Neuroprotection in Parkinson's Disease through Inhibiting NLRP3 Inflammasome; Authors - Guoqing Sun
human	WB	Cell Chem Biol; Identification of KLHDC2 as an efficient proximity-induced degrader of K-RAS, STK33, β-catenin, and FoxP3; Authors - Sascha Röth
mouse	WB	Int J Mol Med; Flavin containing monooxygenase 2 regulates renal tubular cell fibrosis and paracrine secretion via SMURF2 in AKI‑CKD transformation; Authors - Longfei Wang

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C