GAPDH Monoklonaler Antikörper

GAPDH Monoklonal Antikörper für FC (Intra), WB

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hefe, human, Maus, Pflanze, Ratte, Zebrafisch

Anwendung

WB, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen

7

CloneNo.

1E6D9

Kat-Nr. : CL488-60004

Synonyme

G3PD, GAPD, GAPDH, OK/SW cl.12

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Galerie der Validierungsdaten

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  WB analysis using CL488-60004

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-60004 (GAPDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using CL488-60004

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-60004 (GAPDH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using CL488-60004

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GAPDH (CL488-60004, Clone:1E6D9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

CL488-60004 bindet in WB, FC (Intra) GAPDH und zeigt Reaktivität mit Hefe, human, Maus, Pflanze, Ratten, Zebrafisch

• Getestete Reaktivität: Hefe, human, Maus, Pflanze, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GAPDH fusion protein Ag0766
• Vollständiger Name: glyceraldehyde-3-phosphate dehydrogenase
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC004109
• Gene symbol: GAPDH
• Gene ID (NCBI): 2597
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Publikationen

Species	Application	Title
human	WB	Aging (Albany NY); Coagulation factor 2 thrombin receptor promotes malignancy in glioma under SOX2 regulation.; Authors - Guojun Gao
human	WB	Cell Commun Signal; Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction.; Authors - Cefan Zhou
human	WB	Sci Rep; Comparative analysis of pathophysiological parameters between emphysematous smokers and emphysematous patients with COPD.; Authors - Shuang Bai
human	WB	Exp Cell Res; CLEC12B suppresses lung cancer progression by inducing SHP-1 expression and inactivating the PI3K/AKT signaling pathway.; Authors - Decai Chi
rat	WB	Virus Res; Interaction of porcine reproductive and respiratory syndrome virus major envelope proteins GP5 and M with the cellular protein Snapin.; Authors - Julie A Hicks
mouse	WB	Reprod Fertil Dev; Effects of 2,3',4,4',5-pentachlorobiphenyl exposure during pregnancy on DNA methylation in the testis of offspring in the mouse.; Authors - Jian-Mei Zhang

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C