GALC Polyklonaler Antikörper

GALC Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12887-1-AP

Synonyme

Galactocerebrosidase, galactosylceramidase, GALC, GALCERase

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Galerie der Validierungsdaten

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  WB analysis of A375 using 12887-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12887-1-AP (GALC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12887-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12887-1-AP (GALC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12887-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12887-1-AP (GALC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12887-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12887-1-AP (GALC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

12887-1-AP bindet in WB, IHC, ELISA GALC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GALC fusion protein Ag3915
• Vollständiger Name: galactosylceramidase
• Berechnetes Molekulargewicht: 668 aa, 76 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC036518
• Gene symbol: GALC
• Gene ID (NCBI): 2581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galactosylceramidase (Galc) is a lysosomal enzyme involved in the catabolism of galactosylceramide, a major lipid in myelin, kidney, and epithelial cells of the small intestine and colon. The normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits. Mutation of GALC gene will lead to Krabbe disease (globoid cell leukodystrophy), an autosomal recessive neurodegenerative disorder that affects both the central and peripheral nervous system due to an enzymatic defect of galactocerebrosidase.

Publikationen

Species	Application	Title
human	IHC	Chembiochem; A specific activity-based probe to monitor family GH59 galactosylceramidase - the enzyme deficient in Krabbe disease.; Authors - André Ra Marques