GABPB1 Polyklonaler Antikörper

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• KD/KO Validated

GABPB1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 12597-1-AP

Synonyme

GA-binding protein subunit beta-1, E4TF1B, E4TF1 53, E4TF1 47, E4TF1

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 12597-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12597-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12597-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12597-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12597-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12597-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12597-1-AP (GABPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12597-1-AP

  IP result of anti-GABPB1 (IP:12597-1-AP, 4ug; Detection:12597-1-AP 1:1000) with HeLa cells lysate 520ug.

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  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 12597-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12597-1-AP (GABPB1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HeLa using 12597-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GABPB1 antibody (12597-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushodengewebe, Mauslungengewebe, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

12597-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GABPB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GABPB1 fusion protein Ag3208
• Vollständiger Name: GA binding protein transcription factor, beta subunit 1
• Berechnetes Molekulargewicht: 347 aa, 38 kDa
• Beobachtetes Molekulargewicht: 36-45 kDa, 47-53 kDa
• GenBank-Zugangsnummer: BC016910
• Gene symbol: GABPB1
• Gene ID (NCBI): 2553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABPB1, also named as E4TF1B, GABPB, GABPB2, E4TF1-47 and E4TF1-53, is a transcription factor capable of interacting with purine rich repeats (GA repeats). It is necessary for the expression of the Adenovirus E4 gene. GABPB1 has 4 isoforms. The MW-of Beta 1 is 53kd and Beta-2 is 47kd(PMID:9525935). This antibody can recognize all the isoforms.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner.; Authors - Andrew Mancini ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage.; Authors - Alexandra M Amen
human, mouse	WB,IHC,IF	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H ; KD Validated
human	WB	Sci Rep; LncRNA GABPB1-AS1 and GABPB1 regulate oxidative stress during erastin-induced ferroptosis in HepG2 hepatocellular carcinoma cells.; Authors - Wenchuan Qi ; KD Validated
human	WB	Biology (Basel); TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.; Authors - Yanbing Wang
mouse	WB	Brain Res Bull; ZFP36 protects against oxygen-glucose deprivation/reoxygenation-induced mitochondrial fragmentation and neuronal apoptosis through inhibiting NOX4-DRP1 pathway.; Authors - Hengjiang Guo