G3BP2 Monoklonaler Antikörper

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• KD/KO Validated

G3BP2 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2E5G3

Kat-Nr. : 68580-1-Ig

Synonyme

G3BP 2, 2E5G3, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2

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  WB analysis of SCaBER using 68580-1-Ig

  SCaBER cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68580-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HepG2 using 68580-1-Ig

  WB result of G3BP2 antibody (68580-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G3BP2 transfected HepG2 cells.

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  WB analysis of A549 using 68580-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 68580-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HCT 116 using 68580-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68580-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of HeLa using 68580-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (68580-1-Ig, Clone: 2E5G3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HCT 116-Zellen, HepG2-Zellen, HT-29-Zellen, K-562-Zellen, MOLT-4-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: sodium arsenite treated HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

68580-1-Ig bindet in WB, IF/ICC, ELISA G3BP2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: G3BP2 fusion protein Ag9222
• Vollständiger Name: GTPase activating protein (SH3 domain) binding protein 2
• Berechnetes Molekulargewicht: 482aa,54 kDa; 449aa,51 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC011731
• Gene symbol: G3BP2
• Gene ID (NCBI): 9908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

Publikationen

Species	Application	Title
human	WB,IF	Nat Cancer; RIOK1 phase separation restricts PTEN translation via stress granules activating tumor growth in hepatocellular carcinoma; Authors - Fanzheng Meng