Foxp3 Monoklonaler Antikörper

Foxp3 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

CloneNo.

3G3

Kat-Nr. : CL488-65089

Synonyme

forkhead box P3, Foxp3, JM2, scurfin, sf

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  FC experiment of mouse splenocytes using CL488-65089

  1x10^6 mouse splenocytes were intracellularly stained with 0.06 ug CoraLite® Plus 488 Anti-Mouse Foxp3 (CL488-65089, Clone:3G3), and 0.06 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5), and 0.06 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65089 bindet in FC (Intra) Foxp3 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: forkhead box P3
• GenBank-Zugangsnummer: BC132333
• Gene symbol: Foxp3
• Gene ID (NCBI): 20371
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Publikationen

Species	Application	Title
mouse	FC (Intra)	Adv Sci (Weinh); Cancer-Associated Fibroblast-Induced Remodeling of Tumor Microenvironment in Recurrent Bladder Cancer; Authors - Ting Liang