FXYD2 Polyklonaler Antikörper

FXYD2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11198-1-AP

Synonyme

Sodium/potassium-transporting ATPase subunit gamma, FXYD domain-containing ion transport regulator 2, FXYD 2, ATP1G1, ATP1C

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Galerie der Validierungsdaten

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  WB analysis of human kidney using 11198-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11198-1-AP (FXYD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11198-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11198-1-AP (FXYD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11198-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11198-1-AP (FXYD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11198-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11198-1-AP (FXYD2 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, humanes Lebergewebe, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

11198-1-AP bindet in WB, IHC, ELISA, IF FXYD2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FXYD2 fusion protein Ag1676
• Vollständiger Name: FXYD domain containing ion transport regulator 2
• Berechnetes Molekulargewicht: 7 kDa
• Beobachtetes Molekulargewicht: 7-10 kDa
• GenBank-Zugangsnummer: BC013289
• Gene symbol: FXYD2
• Gene ID (NCBI): 486
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FXYD2 (FXYD domain-containing ion transport regulator 2), also known as the gamma-subunit of the NaK-ATPase, belongs to the FXYD family which has been proposed to be the regulators of Na, K-ATPase function by lowering affinities of the system for potassium and sodium. The expression of FXYD2 is most abundant in kidney, while it is also detected in several other tissues like placenta, pancreas, and dorsal root ganglia (DRGs). Three splice variants of FXYD2 have been reported in mouse kidney, namely FXYD2 γa, -γb,and -γc. FXYD2 γa has been identified as a pancreatic beta cell-specific biomarker. This antibody can recognize all three isoforms of FXYD2.

Publikationen

Species	Application	Title
mouse	IHC	Cell; Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair.; Authors - Lu Catherine P CP
mouse	IHC	Front Cell Dev Biol; A Na+/K+ ATPase Pump Regulates Chondrocyte Differentiation and Bone Length Variation in Mice.; Authors - Marta Marchini
rat	IHC	Nutrients; Effect of Dapagliflozin and Magnesium Supplementation on Renal Magnesium Handling and Magnesium Homeostasis in Metabolic Syndrome.; Authors - Hwee-Yeong Ng
mouse	IF	Cell Rep; Tumor vessel co-option probed by single-cell analysis.; Authors - Laure-Anne Teuwen
human	WB,IF	J Immunol Res; Downregulation of FXYD2 Is Associated with Poor Prognosis and Increased Regulatory T Cell Infiltration in Clear Cell Renal Cell Carcinoma; Authors - Zedan Zhang
mouse	WB	Brain Res Bull; Microglial SIX2 suppresses lipopolysaccharide (LPS)-induced neuroinflammation by up-regulating FXYD2 expression; Authors - Xia-Yin Cao